human cd4+ or cd8+ t cell enrichment column kit  (Miltenyi Biotec)

Journal: Cancer Management and Research

Article Title: LOX-1+ PMN-MDSC enhances immune suppression which promotes glioblastoma multiforme progression

doi: 10.2147/CMAR.S210545

Figure Lengend Snippet: LOX-1+ PMN is a population of PMN-MDSC in GBM patient PB. ( A, B ) Suppressive function of LOX-1+ and LOX-1− PMN sorted from PB of GBM patients in allogeneic MLR. The data indicate the mean±sem of three independent experiments. CD4+T cell ( A ) and CD8+ T cell ( B ) proliferation was determined in triplicates using 3H-thymidine uptake. ( C ) Production of ROS in LOX-1+ and LOX-1− PMN from six patients with GBM. Production of ROS was detected by staining with DCFDA. ( D, E ) ARG1 ( D ) and iNOS ( E ) expression in LOX-1- and LOX-1+ PMN from six patients with GBM detected by qPCR. ( F, G ) Effect of 1 μM of N-acetyl L-cysteine (NAC), 1,000 U/mL of catalase and 20 μM nor-NOHA on immune suppressive function of LOX-1+ PMN. The data indicate the mean±sem of three independent experiments. Allogeneic MLR and 1:1 PMN: T cell ratio was used in all experiments. CD4+T cell ( F ) and CD8+ T cell ( G ) proliferation were determined in triplicates by 3H-thymidine incorporation. ** P <0.01, *** P <0.001. Abbreviations: LOX-1, lectin-type oxidized LDL receptor 1; PMN, polymorphonuclear neutrophil; GBM, glioblastoma multiforme; PB, peripheral blood; CPM, counts per minute.

Article Snippet: The isolation of CD4+ or CD8+ T cells from the PBMC of the same patients as LOX-1+ PMN was performed using human CD4+ or CD8+ T Cell Enrichment Column Kit (Miltenyi).

Techniques: Staining, Expressing

Journal: Cancer Management and Research

Article Title: LOX-1+ PMN-MDSC enhances immune suppression which promotes glioblastoma multiforme progression

doi: 10.2147/CMAR.S210545

Figure Lengend Snippet: LOX-1+ PMN is negatively correlation with effector immune cells in GBM patient PB. ( A ) Typical example of the analysis of the proportion of LOX-1+ PMN, IFN-γ+CD4+ T cells and IFN-γ+CD8+ T cells in two patients with GBM. ( B, C ) Quantification showing the associations between LOX-1+ PMN and IFN-γ+CD4+ T cells ( B ) and IFN-γ+CD8+ T cells in 10 patients with GBM ( C ). Abbreviations: LOX-1, lectin-type oxidized LDL receptor 1; PMN, polymorphonuclear neutrophil; GBM, glioblastoma multiforme; PB, peripheral blood.

Article Snippet: The isolation of CD4+ or CD8+ T cells from the PBMC of the same patients as LOX-1+ PMN was performed using human CD4+ or CD8+ T Cell Enrichment Column Kit (Miltenyi).

Techniques:

Journal: Cancer Management and Research

Article Title: LOX-1+ PMN-MDSC enhances immune suppression which promotes glioblastoma multiforme progression

doi: 10.2147/CMAR.S210545

Figure Lengend Snippet: LOX-1 defines a subset of PMN-MDSC in GBM patient tissues. ( A) Typical example of the analysis of PMN in GBM patient tissue. ( B, C ) Suppressive function of LOX-1+ and LOX-1− PMN sorted from tumor tissue of GBM patients in allogeneic MLR. CD4+T cell ( B ) and CD8+ T cell ( C ) proliferation was determined in triplicates using 3H-thymidine uptake. ( D ) Production of ROS in LOX-1+ and LOX-1− PMN from five patients with GBM. Production of ROS was detected by staining with DCFDA. ( E, F ) ARG1 ( E ) and iNOS ( F ) expression in LOX-1- and LOX-1+ PMN from five patients with GBM detected by qPCR. * P <0.05, ** P <0.01, *** P <0.001. Abbreviations: LOX-1, lectin-type oxidized LDL receptor 1; PMN, polymorphonuclear neutrophil; GBM, glioblastoma multiforme; MDSC, myeloid-derived suppressor cell; DCFDA, dichlorodihydrofluorescein diacetate; MLR, mixed lymphocyte reaction; n.s, not significant.

Article Snippet: The isolation of CD4+ or CD8+ T cells from the PBMC of the same patients as LOX-1+ PMN was performed using human CD4+ or CD8+ T Cell Enrichment Column Kit (Miltenyi).

Techniques: Staining, Expressing, Derivative Assay